Single-cell RNA-seq of mouse developing spinal cord
收藏Alliance of Genome Resources2026-08-01 收录
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12 cervicothoracic sections of mouse embryos were dissected at E9.5 (2 replicates), E10.5 (2 replicates), E11.5 (2 replicates), E12.5 (3 replicates), E13.5 (3 replicates). Single-cell RNA-seq libraries were encapsulated, sequenced, aligned, and counted with the 10X Genomics' Chromium platform and Illumina's HiSeq 4000 platform.
本研究共收集12份小鼠胚胎颈胸区段样本，分别于胚胎发育第9.5天（E9.5，2个生物学重复）、第10.5天（E10.5，2个生物学重复）、第11.5天（E11.5，2个生物学重复）、第12.5天（E12.5，3个生物学重复）及第13.5天（E13.5，3个生物学重复）完成解剖分离。依托10X Genomics Chromium平台与Illumina HiSeq 4000测序平台，完成了单细胞RNA测序（single-cell RNA-seq）文库的封装、测序、序列比对及基因计数分析。
