Single Cell RNA-sequencing of cell types isolated by FACS from normal human prostates

Single-cell RNA-sequencing was conducted on specific cell types and heterogeneous viable cells (sorted by FACS) in order to determine a cellular atlas of the normal human prostate. Overall design: Fresh human prostate specimens were digested into single-cell suspensions for flow cytometry, populations of cells were subject to single-cell RNA-sequencing using the 10x Genomics platform.