三代全长转录组分析

该数据是林木次生生长的分子调控和环境胁迫机制项目论文，数据内容是三代全长转录组分析。提出了一种用于Iso-Seq分析的一站式解决方案，称为PRAPI，用于分析替代转录起始（ATI），替代剪接（AS），替代切割和聚腺苷酸化（APA），天然反义转录本（NAT）和环状RNA （circRNA）。PRAPI能够将Iso-Seq全长同工型与短读数据结合在一起，例如RNA-Seq或聚腺苷酸位点测序（PAS-seq），用于NAT，AS，APA和circRNA的差异表达分析。此外，当基因注释可用时，PRAPI可以注释新基因并纠正错误注释的基因。最后，PRAPI生成高质量的矢量图形，以可视化并突出显示Iso-Seq结果。

This dataset is derived from a research paper associated with the project studying molecular regulation and environmental stress response mechanisms of secondary forest tree growth. The core content of this dataset focuses on third-generation full-length transcriptome analysis. This work proposes a one-stop analytical solution named PRAPI for Iso-Seq data processing, which enables the identification of alternative transcription initiation (ATI), alternative splicing (AS), alternative cleavage and polyadenylation (APA), natural antisense transcripts (NATs), and circular RNAs (circRNAs). PRAPI can integrate full-length Iso-Seq isoforms with short-read sequencing datasets such as RNA-Seq or polyadenylation site sequencing (PAS-seq) to perform differential expression analysis of NATs, AS events, APA events, and circRNAs. Additionally, when reference gene annotations are available, PRAPI can annotate novel genes and correct misannotated genes. Finally, PRAPI generates high-quality vector graphics to visualize and highlight Iso-Seq analysis results.

搜集汇总

背景与挑战

背景概述

该数据集是'林木次生生长的分子调控和环境胁迫机制'项目的一部分，专注于三代全长转录组分析，提出并应用了PRAPI一站式解决方案，用于分析转录组中的多种复杂事件，如替代剪接和环状RNA，并支持数据整合和可视化。数据集以文档格式提供，共享级别为协议共享，适用于植物学领域的研究。