Global circulating miRNA screen after morphine delivery

Opioid use is detrimental to bone health, causing both indirect and direct effects on bone turnover. The aim of the study was to develop a mouse model to evaluate the impact of sustained morphine exposure on bone turnover of both male and female mice and to identify candidate miRNA-mediated regulatory mechanisms that could affect bone. We evaluated the effects of sustained morphine treatment on the skeleton, metabolism, and body composition of male and female C57BL/6J mice by treating with vehicle (0.9% saline) or morphine (18 mg/kg) using subcutaneous osmotic minipumps for 25 days.