Chromatin profiling of human mast cells. Chromatin profiling of human mast cells

Here, we characterized the steady state, immunoglobulin E (IgE)-sensitized and anti-IgE-mediated chromatin reorganization in human peripheral blood-derived MCs Overall design: Cells were left untreated or sensitized overnight with myeloma-IgE (0.5 μg/ml) and treated with anti-IgE (1 μg/ml) for 2h. H3K27ac CHIP-seq or H3K4me3 CHIP-seq was performed.