High-resolution Hi-C maps in EV and ZFP661-3HA OE mESCs

In this assay, we aimed to investigate the impacts of ZFP661 on the permeability of CTCF barriers to chromatin interactions. To achieve this, we generated high-resolution Hi-C maps using empty vector (EV) control and ZFP661-3HA overexpressing (OE) mESCs. Our findings demonstrate that ZFP661 suppresses the trapping of cohesin at the CTCF barriers and modulates their permeability. We compared the alteration of insulation scores at CTCF barriers that are co-occupied with ZFP661 and those that are not, and also evaluated the transition between the two types of competitive interactions (being trapped at the CTCF barriers and those passing over them) after overexpressing ZFP661. Each genotype was represented by two biological replicates.