Oocyte-expressed Yes-associated Protein (YAP) is a Key Activator of the Early Zygotic Genome in Mouse

We generated maternal and paternal mouse models with Yap1-deletion, and elucidated the function of maternal YAP in zygotic genome activation. RNA sequencing with 2 WT oocyte samples at GV stage, 2 YAP-/- oocyte samples at GV stage, 1 WT embryo samples at 4cell stage, 1 YAP-/- embryo samples at at 4-cell stage.本研究构建了携带Yap1基因敲除的母系与父系小鼠模型，阐明了母源性YAP在合子基因组激活（zygotic genome activation）中的功能。本次RNA测序涉及的样本包括：2份野生型（Wild Type, WT）GV期（germinal vesicle stage）卵母细胞样本、2份Yap1敲除（Yap1-/-）GV期卵母细胞样本、1份野生型4细胞期胚胎样本，以及1份Yap1敲除4细胞期胚胎样本。