Analysis of EmPlk1 activity in Xenopus oocytes.

Wild-type (wt) and mutant forms (T179D, constitutively active; T179V, non-activatable; wtKD, T179DKD, kinase dead mutants) of EmPlk1 were expressed in Xenopus oocytes (20 oocytes per set) and germinal vesicle breakdown (GVBD) assays were carried out in the presence of different concentrations of BI 2536 as indicated to the left. Treatment by the natural inducer, progesterone (PG), served as a positive control. Displayed are the percentages of Xenopus oocytes that underwent GVBD in the different experimental settings (mean of three independent experiments). ‘-’ indicates that this condition has not been tested.