S1G Figure

S1G Fig. Viral gene expression heterogeneity does not completely explain IFN-β expression. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Cells from the lytic+casp-i samples were collected both without sorting (“bulk”) and after sorting based on tdTomato expression (tdTomato + and -). RNA was extracted and KSHV ORF59 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. The fold enrichment of mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale. Results in this figure were obtained from the same samples used in Fig 1 and are thus directly comparable to IFN-β enrichment shown in those Figure. ns = p>0.05, ** = p<0.01. One-Way ANOVA followed by Tukey’s post hoc multiple comparisons test. n≥ 4 for all experiments.