RNA-seq analysis in mouse cortical extracts with deletion of STI1 TPR1 domain

Long RNA-sequencing was performed on 5 cortex samples from STI1 wt/wt and 5 STI1 dTPR/dTPR mice. RIN values for these samples ranged between 8.2 and 8.7. Illumina sequencing yielded an average of 797,963 reads per sample, with 93.69% reads mapping rate to the mouse genome. We first verified the complete absence of STI1 exons 2 and 3 in the dTPR/dTPR samples compared to the wt/wt control tissues where these axons were both expressed. Principal component analysis and sample distance matrix analyses did not segregate between the two groups, indicating minimal differences between them. Furthermore, thorough RNA sequencing analysis did not reveal any significant changes in transcripts passing FDR correction (padj<0.05). Briefly, there were 85 gene transcripts (representing 18 unique genes) that passed p.value<0.05 and whose levels were changed by more than 1.5 fold increase (or 0.75 decrease) Examination of transcriptome changes in control and transgenic mice lacking STI1-TPR1 domain本数据集对5份STI1野生型纯合（STI1 wt/wt）小鼠与5份STI1 dTPR纯合（STI1 dTPR/dTPR）小鼠的皮层样本开展了长读长RNA测序（Long RNA-sequencing）。所有样本的RNA完整性数（RNA Integrity Number, RIN）介于8.2至8.7之间。Illumina测序（Illumina sequencing）为每个样本平均产出797,963条读段，其中93.69%的读段可比对至小鼠基因组。我们首先验证了：相较于这些外显子均有表达的野生型对照组织，dTPR/dTPR样本中完全缺失STI1外显子2和3。主成分分析（Principal Component Analysis, PCA）与样本距离矩阵分析均未将两组样本区分开，提示两组间差异极小。此外，全面的RNA测序分析未发现任何通过错误发现率（False Discovery Rate, FDR）校正的转录本存在显著表达变化（padj<0.05）。简言之，共有85条基因转录本（对应18个独特基因）满足P值<0.05且表达量上调幅度超过1.5倍，或下调至原水平的0.75倍以下。本研究针对缺失STI1-TPR1结构域的对照与转基因小鼠的转录组变化展开分析。