Precision data on low count DNA ploidy with FxCycle Violet and six color immunophenotyping

To evaluate precision and accuracy of DNA ploidy analysis at very low levels of tumor cells.
Conclusion:
This method DNA ploidy analysis using FxCycle violet and simultaneous six color immunophenotyping can be used flow cytometric DNA ploidy determination in very small number of tumor cells (up to 100 events) with high precision and negligible variation. Hence can be used in rare event analysis.
Notes:
This experiment proves that this method can be useful for DNA ploidy analysis of rare tumor cells. However, it is recommended acquire at least 200 events of tumor cells for good results for rare events of tumor. Although, CV of G1 peak might be slightly higher (between 4 to 6), it is acceptable as it is still falls within the 10% standard error and does not affect the results of the assay. Few unstained and apoptotic cells always present in the sample showing decreasing staining pattern of FxCycle. Hoeweer, these does not interfere with analysis and interpretation once gated out. DNA ploidy and simultaneous six color immunophenotyping using FxCycle-Violet.