Pluripotency-associated miR-290/302 family of microRNAs promote the dismantling of naive pluripotency

Upon RNA profiling of WT and Dgcr8-/- mESCs in 2iL and differentiation for 48 hours, miRNAs are proved to be important for the transcriptional changes during ESC exiting from pluripotency. Further miRNA sequencing of WT ESCs in different timepoints revealed that miRNAs expression changes dynamically during ESC to EpiLC transition and identified miR-290/302 as key miRNAs facilitating the dismantling of naive pluripotency. Total RNA was analyzed from WT and Dgcr8-/- mESCs and Dgcr8 in 2iL and differentiation for 48 hours. Small RNA was sequenced for WT mESCs in 4 timepoints during exiting from pluripotency.Two biological replicates were sequenced for each sample.