Derivation of hypoblast stem cells from rat embryonic stem cells.[RNA-seq]

We report the mRNA profiles of single cells with high-throughput sequencing (RNA-seq) of hypoblast stem cells derived from rat embryonic stem cells. The single-cell data was combined with the previously published dataset: transcriptomes of mouse embryo cells (inner cell mass or trophectoderm). The clustering revealed that rat embryonic stem cell-derived hypoblast stem cells were similar to the mouse inner cell mass cells.本研究通过高通量测序（high-throughput sequencing, RNA-seq）获取了大鼠胚胎干细胞来源的下胚层干细胞（hypoblast stem cells）的单细胞mRNA表达谱。本研究将该单细胞测序数据与已发表的小鼠胚胎细胞转录组（transcriptomes）数据集进行整合，该数据集涵盖内细胞团（inner cell mass）与滋养外胚层（trophectoderm）两类细胞的转录组信息。聚类分析（clustering）结果显示，大鼠胚胎干细胞来源的下胚层干细胞与小鼠内细胞团细胞的转录组特征高度相似。