Transcriptome analysis revealed the genes involved in cold stress during flowering from Prunus sibirica

Through transcriptome sequencing analysis of clones with strong freezing resistance (CR_453) and weak freezing resistance (NC_371) of Prunus sibirica，a total of 15,228significantly differentially expressed genes were obtained. Among them, there are 6,630 up-regulated genes and 8,598 down-regulated genes. Differential genes are compared and screened, and B3, MYB, AAA, zf-GRF, bHLH, TCP, WD40, HSP, PPR, Zim, AP2, CBF, etc, Freeze-related gene family.It is also found that a large number of gene families are regulated by hormones, including GST_N、Auxin_inducible， Abhydrolase_1，P450，AP2，ADH_N，Zim，HATPase_c，2OG-FeII_Oxy，IPPT，C2，RRM_1and so on. Using Prunus sibirica flower organs with strong frost resistance clone No. 453 (treatment group) and weak frost resistance clone No. 371 (control group) as the test materials, flowers were collected at 0 h, 15min, 30min, 1h, 2h when the flower organs were subjected to frost (-4 °C). The clones were repeated 3 times, placed in liquid nitrogen, stored in an ultra-low temperature refrigerator (-80 °C), and transcriptome sequencing was completed by Nuovo Zhiyuan (Beijing) Co., Ltd.