complete list of clinical cases with clinical data at radical RP and final pathological or molecular classification with Leave-One-Out cross validation procedure in order to obtain a unique classification for each sample.

Mean age of the patients was 66.8±5.7. Mean PSA value at RP was 10.2±8.7 ng/mL. Gleason score is given by examination of the whole gland (fixed and embedded) after RP. Pathological evaluation of the frozen sections is indicated, together with percent of cancer cells given as a mean of pre- and post-RNA frozen sections. 44/66 were CaP-benign paired specimens obtained from the same patient. Specimens misclassified by the molecular method are in bold italic.