Expression patterns of white blood cells in response to individual food treatments relative to LPS treatment and untreated in an FPIES subject.

Total blood white blood cells from a FPIES subject were treated with individual treatmens of 2 foods that were safe for the subject (pear and breast milk), two foods that were triggers (quinoa and sweet potato), and 19 foods of unknown status for the subject. These treatments were compared to LPS treatment and untreated. Blood was drawn from FPIES subject who was on trigger-food avoidance, the WBCs isolated by ACK buffer RBC lysis, and the WBCs plated in 12-well plate wells, and treated with individual food treatments, LPS or untreated.