circRNA Microarray Service for 6 mouse samples

We have completed the Arraystar Mouse circRNA Array v2 analysis of the 6 samples that you submitted. Total RNA from each sample was quantified using the NanoDrop ND-1000. The sample preparation and microarray hybridization were performed based on the Arraystar’s standard protocols. Briefly, total RNAs were digested with Rnase R (Epicentre, Inc.) to remove linear RNAs and enrich circular RNAs. Then, the enriched circular RNAs were amplified and transcribed into fluorescent cRNA utilizing a random priming method (Arraystar Super RNA Labeling Kit; Arraystar). The labeled cRNAs were hybridized onto the Arraystar Mouse circRNA Array v2 (8x15K, Arraystar). After having washed the slides, the arrays were scanned by the Agilent Scanner G2505C. Six samples were included and divided into two groups. One group was exposed to 3% sevoflurane for a single 4-hour period at 7 days postpartum in mice, while the other group was not exposed to sevoflurane.