Crassostrea gigas high-throughput bisulfite sequencing (larvae and sperm tissues)

This fileset contains genomic feature tracks from high-throughput bisulfite sequencing for Pacific oyster (Crassostrea gigas) spermatozoa and larvae samples. Samples were taken from two males (Male 1 and Male 3) and larvae taken from these males at Day 3 (D3) and Day 5 (D5) post-fertilization. Feature tracks were developed to be viewed with Integrative Genomics Viewer (http://www.broadinstitute.org/igv/) in conjunction with the C. gigas genome (Ensembl). File descriptions:<br><em>methratio_out_CG5x_IGV_M1.igv</em> - Male 1 location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>methratio_out_CG5x_IGV_M3.igv</em> - Male 3 location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>methratio_out_CG5x_IGV_T1D3.igv</em> - Larvae from male 1 day 3 post-fertilization location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>methratio_out_CG5x_IGV_T1D5.igv </em>- Larvae from male 1 day 5 post-fertilization location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>methratio_out_CG5x_IGV_T3D3.igv</em> - Larvae from male 3 day 3 post-fertilization location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>methratio_out_CG5x_IGV_T3D5.igv</em> - Larvae from male 3 day 5 post-fertilization location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>LarvaeSperm_igv.xml</em> - A session file, which loads sample methylation tracks and location of C.gigas genome features.

本数据集涵盖源自太平洋牡蛎（*Crassostrea gigas*，学名）精子与幼虫样本的高通量亚硫酸氢盐测序基因组特征轨道（genomic feature tracks）。样本采集自2只雄性个体（雄性1号与雄性3号），并包含对应雄性个体在受精后第3天（D3）、第5天（D5）的子代幼虫样本。上述基因组特征轨道可配合太平洋牡蛎参考基因组（基于Ensembl数据库构建），通过整合基因组浏览器（Integrative Genomics Viewer，简称IGV，访问链接：http://www.broadinstitute.org/igv/）进行可视化查看。
文件详情如下：
- `methratio_out_CG5x_IGV_M1.igv`：对应雄性1号样本，记录所有测序深度不低于5x的已分析CpG二核苷酸（CpG dinucleotides）的甲基化位点位置与甲基化比例。
- `methratio_out_CG5x_IGV_M3.igv`：对应雄性3号样本，记录所有测序深度不低于5x的已分析CpG二核苷酸的甲基化位点位置与甲基化比例。
- `methratio_out_CG5x_IGV_T1D3.igv`：对应雄性1号受精后第3天的幼虫样本，记录所有测序深度不低于5x的已分析CpG二核苷酸的甲基化位点位置与甲基化比例。
- `methratio_out_CG5x_IGV_T1D5.igv`：对应雄性1号受精后第5天的幼虫样本，记录所有测序深度不低于5x的已分析CpG二核苷酸的甲基化位点位置与甲基化比例。
- `methratio_out_CG5x_IGV_T3D3.igv`：对应雄性3号受精后第3天的幼虫样本，记录所有测序深度不低于5x的已分析CpG二核苷酸的甲基化位点位置与甲基化比例。
- `methratio_out_CG5x_IGV_T3D5.igv`：对应雄性3号受精后第5天的幼虫样本，记录所有测序深度不低于5x的已分析CpG二核苷酸的甲基化位点位置与甲基化比例。
- `LarvaeSperm_igv.xml`：会话配置文件，用于加载样本甲基化轨道及太平洋牡蛎基因组特征的位置信息。

搜集汇总

背景与挑战

背景概述

该数据集包含太平洋牡蛎精子和幼虫样本的高通量亚硫酸氢盐测序数据，用于分析CpG二核苷酸的甲基化位置和比例，覆盖度大于5倍。数据以基因组特征轨道形式提供，适用于整合基因组查看器（IGV），旨在研究牡蛎发育过程中的表观遗传变化，如DNA甲基化在配子发生和早期发育中的作用。