Crassostrea gigas high-throughput bisulfite sequencing (larvae and sperm tissues)

资源简介：

This fileset contains genomic feature tracks from high-throughput bisulfite sequencing for Pacific oyster (Crassostrea gigas) spermatozoa and larvae samples. Samples were taken from two males (Male 1 and Male 3) and larvae taken from these males at Day 3 (D3) and Day 5 (D5) post-fertilization. Feature tracks were developed to be viewed with Integrative Genomics Viewer (http://www.broadinstitute.org/igv/) in conjunction with the C. gigas genome (Ensembl). File descriptions:<br><em>methratio_out_CG5x_IGV_M1.igv</em> - Male 1 location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>methratio_out_CG5x_IGV_M3.igv</em> - Male 3 location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>methratio_out_CG5x_IGV_T1D3.igv</em> - Larvae from male 1 day 3 post-fertilization location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>methratio_out_CG5x_IGV_T1D5.igv </em>- Larvae from male 1 day 5 post-fertilization location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>methratio_out_CG5x_IGV_T3D3.igv</em> - Larvae from male 3 day 3 post-fertilization location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>methratio_out_CG5x_IGV_T3D5.igv</em> - Larvae from male 3 day 5 post-fertilization location and proportion of methylation for all analyzed CpG dinucleotides with greater than 5x coverage. <em>LarvaeSperm_igv.xml</em> - A session file, which loads sample methylation tracks and location of C.gigas genome features.

本数据集涵盖源自太平洋牡蛎（*Crassostrea gigas*，学名）精子与幼虫样本的高通量亚硫酸氢盐测序基因组特征轨道（genomic feature tracks）。样本采集自2只雄性个体（雄性1号与雄性3号），并包含对应雄性个体在受精后第3天（D3）、第5天（D5）的子代幼虫样本。上述基因组特征轨道可配合太平洋牡蛎参考基因组（基于Ensembl数据库构建），通过整合基因组浏览器（Integrative Genomics Viewer，简称IGV，访问链接：http://www.broadinstitute.org/igv/）进行可视化查看。文件详情如下： - `methratio_out_CG5x_IGV_M1.igv`：对应雄性1号样本，记录所有测序深度不低于5x的已分析CpG二核苷酸（CpG dinucleotides）的甲基化位点位置与甲基化比例。 - `methratio_out_CG5x_IGV_M3.igv`：对应雄性3号样本，记录所有测序深度不低于5x的已分析CpG二核苷酸的甲基化位点位置与甲基化比例。 - `methratio_out_CG5x_IGV_T1D3.igv`：对应雄性1号受精后第3天的幼虫样本，记录所有测序深度不低于5x的已分析CpG二核苷酸的甲基化位点位置与甲基化比例。 - `methratio_out_CG5x_IGV_T1D5.igv`：对应雄性1号受精后第5天的幼虫样本，记录所有测序深度不低于5x的已分析CpG二核苷酸的甲基化位点位置与甲基化比例。 - `methratio_out_CG5x_IGV_T3D3.igv`：对应雄性3号受精后第3天的幼虫样本，记录所有测序深度不低于5x的已分析CpG二核苷酸的甲基化位点位置与甲基化比例。 - `methratio_out_CG5x_IGV_T3D5.igv`：对应雄性3号受精后第5天的幼虫样本，记录所有测序深度不低于5x的已分析CpG二核苷酸的甲基化位点位置与甲基化比例。 - `LarvaeSperm_igv.xml`：会话配置文件，用于加载样本甲基化轨道及太平洋牡蛎基因组特征的位置信息。