Discovery of lncRNA biomarkers of prostate cancer

In the last decade, new high-throughput sequencing techniques have revealed the complexity of the human transcriptome, allowing the characterization of long non-coding (lnc)RNAs. Since their expression has been reported as very specific to tissue, developmental stage and pathological variations, some lncRNAs have been proposed as biomarkers for diagnosis as well as prognosis of tumors. In this project, we aim to build an exhaustive catalogue of long non-coding RNAs and isolate those which allow detection and risk assessement of prostate cancer. For this purpose we performed a high throughput total stranded RNA-sequencing of 24 samples (8 normal and 16 tumor tissues). Overall design: Total RNA was extracted from biopsy specimens, checked for the quality and than converted into cDNA for total stranded RNA-sequencing.