STARR-seq based mutagenesis of transcription factor binding sites

We performed a STARR-seq based, massively parallel reporter assay on reference genome and mutation-containing oligonucleotide sequences from regions associated with a large number of transcription factor ChIP-seq peaks or DHS-footprinted motifs. The data set includes a plasmid DNA oligo library reference sample and 6 replicate RNA samples isolated from the HepG2 cell line. 92,000 170bp oligonucleotides were cloned in parallel into the STARR-seq reporter vector and transfected into HepG2 in 6 replicates. RNA was harvested from these 6 replicates after 48 hours and oligonucleotide expression levels were compared to their representation in the original plasmid DNA pool.