Immunofluorescence staining against specific histone modifications and live MSL3-GFP signal have different width and distribution on mitotic chromosomes.

Rows from top to bottom: number of chromosomes measured for each data set; mean chromosome width after DAPI staining for the antibody stained chromosomes or for His2AvDmRFP1-labeling for live chromosomes in MSL3-GFP-expressing tissues; mean immunofluorescence (IF) staining width (the first three columns) or MSL3-GFP signal width (the rightmost column) for live cells; individually normalized and averaged widths of antibody or MSL3-GFP labels, “IF” is an antibody staining width, “DNA” is a chromosome width; average separation of centers of peripheral antibody or MSL3-GFP signals across the chromosome width (summarized in Figure S5 and more details can be found in Text S1).