Dataset for article Self-Assembled PEG-Based Fluorosomes for Cellular Internalization

--------------------------------------------------------------------------------------------------------------------------- Dataset for article Self-Assembled PEG-Based Fluorosomes for Cellular Internalization --------------------------------------------------------------------------------------------------------------------------- ReadMe version: 1.0 (2026-06-25) Dataset version: 1.0 (2026-06-25) Dataset DOI: 10.5281/zenodo.21227471 Related article: Leana Vratovic, Vit Guiglielmo Misurec, Martina Kabesova, Ewa Pavlova, Jan Kucka, Jan Pankrac, Ludek Sefc, Martin Hruby, and Miroslav Vetrik, Self-Assembled PEG-Based Fluorosomes for Cellular Internalization, ACS Applied Nano Materials 9 (2026) 4095-4108, https://doi.org/10.1021/acsanm.5c05222 -------------------------------------------------------------------- CONTACT -------------------------------------------------------------------- Miroslav Vetrik [e-mail] ORCID: 0000-0002-0118-3799 Phone: [phone] Institute of Macromolecular Chemistry, Czech Academy of Sciences (IMC CAS) Heyrovsky sq. 2, 162 06 Prague 6, Czech Republic Secondary repository/scientific contact: Martin Hruby, [e-mail]; [e-mail], ORCID: 0000-0002-5075-261X, IMC CAS ______Creators______ Leana Vratovic (ORCID: 0009-0006-7601-1541; IMC CAS), majority of experimental work; data curation Vit Guiglielmo Misurec (ORCID 0000-0002-7183-6281; IMC CAS), NMR measurements; acquisition and validation of analytical data Martina Kabesova (First Faculty of Medicine, Charles University), biological experiments; biological-data interpretation Ewa Pavlova (ORCID 0000-0002-6693-9040; IMC CAS), TEM experiments; TEM data analysis Jan Kucka (ORCID 0000-0003-2489-5315; IMC CAS), intravenous administration to experimental animals; assistance with in vivo procedures Jan Pankrac (ORCID 0000-0002-9574-1391; CAPI, First Faculty of Medicine, Charles University), in vivo evaluations; analysis of biological results Ludek Sefc (ORCID 0000-0003-2846-220X ; CAPI, First Faculty of Medicine, Charles University), consultation related to in vivo studies; funding acquisition Martin Hruby (ORCID: 0000-0002-5075-261X; IMC CAS), consultation; funding acquisition Miroslav Vetrik (ORCID: 0000-0002-0118-3799; IMC CAS), conceptualization; experimental design; supervision; funding acquisition -------------------------------------------------------------------- DATA AVAILABILITY AND ACCESS INSTRUCTIONS -------------------------------------------------------------------- The dataset is openly accessible under the DOI listed above. The license and terms of reuse are shown in the following chapter below. -------------------------------------------------------------------- LICENSE -------------------------------------------------------------------- ______ReadMe file license______ ReadMe by Martin Hruby, Miroslav Vetrik, Leana Vratovic, and co-authors is licensed under CC BY 4.0 License information: https://creativecommons.org/licenses/by/4.0/ ______Dataset license______ Dataset for article Self-Assembled PEG-Based Fluorosomes for Cellular Internalization by Leana Vratovic, Vit Guiglielmo Misurec, Martina Kabesova, Ewa Pavlova, Jan Kucka, Jan Pankrac, Ludek Sefc, Martin Hruby, and Miroslav Vetrik is licensed under CC BY 4.0 License information: https://creativecommons.org/licenses/by/4.0/ -------------------------------------------------------------------- DESCRIPTION AND METHODOLOGY -------------------------------------------------------------------- ______About the dataset______ Fluorinated amphiphiles are investigated in the related article as self-assembling PEG-based nanocarriers ("fluorosomes") for cellular internalization. The study synthesized a tunable series of monodisperse PEG11 amphiphiles bearing a heptadecafluorinated tail and different terminal groups: amine, glutamine, glutamic acid, succinic acid, folic acid, FITC, and Cy7. These amphiphiles form nanoparticles in aqueous PBS, with terminal-group-dependent surface charge, size, and morphology. The dataset supports the article conclusions using chemical characterization, colloidal characterization, microscopy, cell studies, and animal pilot biodistribution data. It includes NMR and FTIR information for synthesized compounds, CMC and pKa calculations, DLS/ELS-derived nanoparticle characteristics, TEM images, confocal microscopy images and colocalization metrics, flow-cytometry uptake summaries, XTT cell-viability data, hemolysis/biocompatibility figure material where available, and in vivo NIR fluorescence imaging materials. Key article-level nanoparticle values reported from the supplied study include CMC values of approximately 16.2 +/- 5.0 uM for F-PEG-NH2, 22.9 +/- 9.2 uM for F-PEG-Gln, 81.9 +/- 9.6 uM for F-PEG-GA, 30.0 +/- 9.7 uM for F-PEG-SA, 32.8 +/- 7.9 uM for F-PEG-FA, and 19.8 +/- 3.6 uM for F-PEG-FITC. DLS hydrodynamic diameters reported in the article range from about 7 nm for F-PEG-FITC to about 275 nm for F-PEG-SA, with zeta-potential varying from positive for the amine derivative to negative for succinate/FITC derivatives. ______Ethics______ The study includes cell-culture data, murine RBC hemolysis/biocompatibility material as summarized in the article/SI, and murine in vivo fluorescence imaging materials. All in vivo procedures were approved by the Institutional Animal Care and Use Committee and by the Ministry of Education, Youth and Sports of the Czech Republic under approval MSMT-46307/2020-7. ______Sample preparation______ The common fluorinated PEG scaffold was prepared from tert-butyl (35-amino-3,6,9,12,15,18,21,24,27,30,33-undecaoxapentatriacontyl)carbamate and 4,4,5,5,6,6,7,7,8,8,9,9,10,10,11,11,11-heptadecafluoroundecanoyl chloride in THF with triethylamine at 0 deg C to room temperature, followed by deprotection to give the amine/TFA salt 17F-PEG-NH3+ TFA- (compound 1). This compound served as precursor for the end-group derivatives. Compounds were then prepared as follows: (2) F-PEG-Gln by coupling with Fmoc-Gln(Trt)-OH followed by deprotection; (3) F-PEG-GA by coupling with Fmoc-Glu(OtBu)-OH followed by deprotection; (4) F-PEG-SA by reaction with succinic anhydride; (5) F-PEG-FA by coupling with folic acid; (6) F-PEG-FITC by reaction with fluorescein isothiocyanate; and (7) F-PEG-Cy7 by reaction with Cyanine 7 NHS ester. Full reagent amounts and purification details are in the attached Supporting Information. Self-assembled nanoparticles were generally prepared by dissolving amphiphiles in PBS pH 7.4 at 1 mg/mL, stirring at 25 deg C until dissolved, sonicating for 15 min in a Bandelin SONOREX SUPER bath, and leaving the dispersions overnight to self-assemble. For fluorescence uptake and confocal microscopy, mixtures generally contained 97 mol % of the non-fluorescent amphiphile and 3 mol % F-PEG-FITC. For whole-body in vivo imaging, the Cy7-labeled amphiphile was mixed into the nanoparticle formulation at 20 mol % relative to carrier to improve NIR signal. Sample-code mapping used in the supplied archive: F-PEG-NH2 = compound (1) = 17F-Peg11_NH2 = 17F-NH2; F-PEG-Gln = compound (2) = 17F-Peg11_Gln = 17F-Gln = AK_21_17F-PEG-GLn; F-PEG-GA = compound (3) = 17F-Peg11_GluA = 17F-GluA; F-PEG-SA = compound (4) = 17F-Peg11_SucA = 17F-SucA; F-PEG-FA = compound (5) = 17F-Peg11_FA = 17FP-FA = AK_22_17F-PEG-Folic acid; F-PEG-FITC = compound (6) = 17F-Peg11_FITC = 17F-FITC = AK_23_17F-PEG-FITC; F-PEG-Cy7 = compound (7), used principally in in vivo NIR fluorescence imaging. ______Methods of data collection______ *NMR spectra Instrument: Bruker DPX 300 spectrometer according to the article; the Bruker audit-trail files in the ZIP identify TopSpin 4.0.6 and an Avance system for the supplied raw folders. Measurements: 1H and 19F NMR spectra were recorded in deuterated DMSO-d6 and/or CDCl3 at 298 K for structural characterization of fluorinated PEG derivatives. The supporting information also gives representative spectra for precursors and compounds (1)-(6). Archive content: Bruker raw acquisition folders contain fid, acqu/acqus, pulseprogram, audit-trail files, and pdata/1 processed spectra. Top-level assigned NMR PNGs are also supplied for PEG_NMR_CDClassigned2(1).png and PEG_NMR_DMSOassigned(1).png. *FTIR spectra Instrument: PerkinElmer Spectrum 2 spectrometer according to the article. Archive content: FTIR of 17FPeg11-NH2(2).opju is an OriginPro project estimated to contain imported FTIR spectra/figure data. The supporting information reports absorption bands in cm^-1 at maximum intensity for compounds (1)-(6) and precursors. *ESI mass spectrometry Instrumentation according to the article: Waters quadrupole mass spectrometer or Thermo Scientific LCQ Fleet ion trap LC/MS system. Archive content: no separate native MS raw files were identified in merge aaa.zip. MS m/z values are documented in the attached Supporting Information and are therefore not described as separate raw-data files here unless the depositor later adds them. *Nanoparticle preparation and self-assembly Nanoparticles were prepared at 1 mg/mL in PBS pH 7.4, filtered through 0.45 um PTFE syringe filters for DLS/ELS where described, and equilibrated before measurements. In cell experiments, fluorescent formulations used 3 mol % F-PEG-FITC with 97 mol % of the corresponding unlabeled amphiphile. In vivo NIR formulations used F-PEG-Cy7. *Potentiometric titration / apparent pKa Instrument/conditions: calibrated glass electrode HI 3512, Hanna Instruments; 5 mM nanoparticles in 0.1 M NaCl; 25 deg C; 0.1 M NaOH added in 20 uL steps under stirring. Archive content: pKa of NH2.xlsx and Pka of nanoparticles,opju.opju contain titration and/or processed pKa data. The article reports apparent pKa/pI values from inflection points in dpH/dV curves. *Critical micelle concentration (CMC) Method: fluorescein-probe method in PBS pH approximately 7.4 at 25 deg C. Fluorescein concentration was 2.5 x 10^-5 M. Samples equilibrated for 24 h before fluorescence emission spectra were recorded from 450 to 750 nm on a BioTek Synergy H1 Multimode Microplate Reader (Agilent). Archive content: CMC/CMC data.xlsx contains intersection and error calculations; CMC/1.opju to CMC/5.opju are OriginPro project files with curve/plot data. Units are amphiphile concentration (uM) and fluorescence intensity (a.u.). Dynamic light scattering and electrophoretic light scattering (DLS/ELS) Instrument: Zetasizer Nano-ZS ZEN3600, Malvern Instruments, with He-Ne 633 nm laser. Conditions: samples in 1x PBS pH 7.4 at 1 mg/mL, filtered through 0.45 um PTFE syringe filters and equilibrated before measurement; measurements performed in triplicate; DLS hydrodynamic diameters evaluated using the REPES algorithm; zeta-potentials derived from the Smoluchowski model. Archive content: DLS of nanoparticles new for article figures supporting info.opju is an OriginPro project estimated to contain imported DLS/ELS results, summary plots, and figure data. Native Zetasizer .dts/.zdp files were not identified in the supplied ZIP. *Transmission electron microscopy (TEM) Instrument: FEI Tecnai G2 Spirit Twin microscope operated at 120 kV in bright-field mode. Grid preparation: 2 uL nanoparticle dispersion on a 400-mesh copper grid with carbon support film; after 1 min, excess liquid removed; negative staining with 2 wt % uranyl acetate for 30 s; air drying before imaging. Archive content: TEM/ contains PNG/TIF micrographs and processing files for 17F-Peg11_FA, 17F-Peg11_FITC, 17F-Peg11_Gln, 17F-Peg11_GluA, 17F-Peg11_NH2, and 17F-Peg11_SucA. TEM_D81-16.pptx contains assembled TEM figure/slide material. *Confocal microscopy and colocalization Instrument: Olympus LSM FV1200 IX83 confocal microscope according to the article. Conditions: cells grown in 35-mm glass-bottom dishes; fluorescent nanoparticle systems prepared with 97 mol % of compound (1)-(5) and 3 mol % compound (6); cells incubated at approximately 45 uM total amphiphile for 8 h at 37 deg C; DAPI and ER-Tracker Red used for nucleus and endoplasmic-reticulum staining in colocalization experiments. Archive content: 17F-Peg11 with dapi and probe/ contains Olympus .oib files and exported TIFF images. Coloc_KeyMetrics_with_FITC.xlsx contains Pearson, Manders, thresholded Manders, and Costes P values computed using Fiji/ImageJ Coloc2. *Flow cytometry uptake analysis Instrument: NovoCyte Pentheon flow cytometer (Agilent), FITC Ex/Em approximately 488/530 nm, with living cells analyzed after exclusion of propidium iodide-positive cells. Conditions: cells were incubated for 4 or 24 h with approximately 45 uM total amphiphile; each formulation contained 3 mol % F-PEG-FITC and 97 mol % unlabeled amphiphile. Archive content: 20221201_FACS_bez fotek.xls, in vitro data/FACS_clanek.xlsx, and in vitro data/FACS_clanek_Uprava.xlsx contain exported/summarized MFI data by cell line. The Excel sheets reference .fcs source filenames, but native .fcs files were not identified in the supplied ZIP. *XTT cell viability assay Instrument: Tecan Infinite microplate reader for absorbance at 450 nm with 650 nm reference according to the article. Conditions: MCF-7, MDA-MB-231, Panc02, 4T1, EL-4, and LL-2 cells cultured in DMEM with 10% FBS and supplements; cells seeded at 5 x 10^3 cells/well; nanoparticle concentrations 7.8-250 uM; 72 h incubation; XTT reaction for 2 h; viability expressed relative to untreated control. Archive content: Bio/XTT_Souhrn_article.xlsx, in vitro data/XTT_Souhrn_article.xlsx, and Bio/Bio relative viability new for article correction.opju contain raw/processed absorbance-derived cell viability data and plots. *Hemolysis / biocompatibility assay Method: murine RBCs obtained by retro-orbital puncture, diluted in PBS; nanoparticles tested at 15 and 30 uM for 60 min at 37 deg C; hemoglobin release measured at 405 nm on an Infinite F50 microplate reader; 0.1% Triton X-100 and PBS used as positive/negative controls. Archive content: Figure S8 in the supporting information reports hemolytic activity. A separate clearly named hemolysis raw-data workbook was not identified in merge aaa.zip; the relevant source data may be embedded in the OriginPro/biological workbooks or may need to be added separately if required by the repository. *In vivo biodistribution / NIR fluorescence imaging Instrument: Xtreme optical imaging system, Bruker. Conditions: male nude mice (Crl: CD1-Foxn1nu), 13 weeks old; formulations injected via tail vein under isoflurane anesthesia; images acquired at 10 min, 1 h, 2 h, 4 h, 24 h, and 48 h post-injection; excitation 750 nm and emission 830 nm for Cy7. The article describes this as a pilot whole-body imaging study and also describes plasma fluorescence measurements for selected formulations. Archive content: pankrac_clanek/ contains in vivo-method and result notes, assembled PNG/PSD in vivo tables, and figure material. The text files contain early draft method/result notes; where they contain placeholders, the final article values are used in this ReadMe. *ITC/control data Archive content: ITC of F-Peg-OMe/ contains LV ITC to send.zip and LV ITC.docx. The attached article does not prominently describe ITC as a main method, so this folder is treated as auxiliary/control data related to F-Peg-OMe unless the depositor provides more specific metadata. ______Methods of data processing______ The dataset contains raw instrument files where available, exported image files, OriginPro project files, and Excel workbooks with summaries and calculations. For NMR, Bruker acquisition data (fid, acqu/acqus, pulseprogram) and processed pdata/1 folders were generated in TopSpin 4.0.6 according to the archive audit trails. Published NMR assignments and selected spectra are also present in the supporting information and PNG exports. For FTIR, DLS/ELS, CMC, pKa, and some biological plots, OriginPro .opju project files appear to store imported data, calculations, and prepared figures. Exact OriginPro version was not recorded in the attached materials; use a recent OriginPro version capable of opening .opju files. For CMC, values were estimated from the intersection of two linear regions of fluorescein intensity vs amphiphile concentration. CMC/CMC data.xlsx includes slopes, intercepts, and error calculations. For DLS/ELS, hydrodynamic diameter, PDI, and zeta-potential values were calculated as described in the article using the REPES algorithm for DLS and the Smoluchowski model for zeta-potential. For confocal microscopy, images were analyzed in Fiji/ImageJ with the Coloc2 plugin after conversion of fluorescence channels to 32-bit float. Reported outputs include Pearson correlation coefficients, Manders overlap coefficients, thresholded Manders values, and Costes randomization P value. For flow cytometry, data were analyzed with FlowJo according to the article; the supplied Excel workbooks contain exported mean/median FITC/B525-A fluorescence values and fold-change summaries. For XTT, absorbance was blank-subtracted and expressed as percent viability relative to untreated controls. OriginPro and Excel files contain plotted or summarized viability outputs. For in vivo imaging, image analysis used Fiji/ImageJ. Liver and other region-of-interest signals were extracted from fluorescence images after camera offset and flat-field corrections according to the article. -------------------------------------------------------------------- DATASET STRUCTURE -------------------------------------------------------------------- 000_ReadMe.txt 001_Data.zip/ |-- 17F-Peg11 with dapi and probe/ - confocal microscopy raw Olympus .oib files and exported TIFFs for 17F-FITC, 17F-Gln, 17F-GluA, 17F-NH2, 17F-SucA; includes subfolder 17FP-FA/ for folic-acid derivative images |-- 20221201_FACS_bez fotek.xls - legacy/exported flow-cytometry summary workbook, probably without embedded photos despite the Czech phrase "bez fotek" (= without pictures) |-- Bio/ | |-- Bio relative viability new for article correction.opju - OriginPro viability/biological-figure project | |-- XTT_Souhrn_article.xlsx - XTT cell-viability summary workbook |-- CMC/ | |-- 1.opju; 2.opju; 3.opju; 4.opju; 5.opju - OriginPro CMC curve/plot projects | |-- CMC data.xlsx - CMC calculations, line fits, intersections, and error estimates |-- Coloc_KeyMetrics_with_FITC.xlsx - confocal colocalization metrics with FITC/ER marker channels |-- DLS of nanoparticles new for article figures supporting info.opju - OriginPro DLS/ELS summary and supporting-information figure project |-- FTIR of 17FPeg11-NH2(2).opju - OriginPro FTIR spectra/figure project |-- in vitro data/ | |-- FACS_clanek.xlsx - flow-cytometry uptake summaries by cell line | |-- FACS_clanek_Uprava.xlsx - edited/processed flow-cytometry uptake summary workbook | |-- XTT_Souhrn_article.xlsx - XTT viability workbook for 4T1, EL4, LL2, Panc02 and graphs |-- ITC of F-Peg-OMe/ | |-- LV ITC to send.zip - nested archive with ITC/control data as supplied | |-- LV ITC.docx - ITC/control data notes |-- NMR/ | |-- AK_21_17F-PEG-GLn odchraneny_Necisteny/ - Bruker NMR raw and processed folders for F-PEG-Gln; includes experiment 43 and 50- siteny II x | |-- AK_22_17F-PEG-Folic acid/ - Bruker NMR raw and processed folder for F-PEG-FA; includes experiment 44 | |-- AK_23_17F-PEG-FITC/ - Bruker NMR raw and processed folder for F-PEG-FITC; includes experiment 45 |-- pankrac_clanek/ | |-- metodika.txt - draft in vivo methods notes | |-- vysledky.txt - draft in vivo results notes | |-- cast 2/4 groups table new ver.png - assembled in vivo image/table figure | |-- tabule_invivo/4 groups table.png; 4 groups table.psd; tabule_popis.txt - in vivo table/figure source and description |-- PEG_NMR_CDClassigned2(1).png; PEG_NMR_DMSOassigned(1).png - assigned NMR spectrum images |-- Pka of nanoparticles,opju.opju - OriginPro pKa/titration project |-- pKa of NH2.xlsx - pKa/titration spreadsheet, 404 rows x 15 columns in Sheet1 |-- TEM/ | |-- 17F-Peg11_FA/ - TEM images and processing files for folic-acid derivative | |-- 17F-Peg11_FITC/ - TEM images and processing files for FITC derivative | |-- 17F-Peg11_Gln/ - TEM images and processing files for glutamine derivative | |-- 17F-Peg11_GluA/ - TEM images and processing files for glutamic-acid derivative | |-- 17F-Peg11_NH2/ - TEM images and processing files for amine derivative | |-- 17F-Peg11_SucA/ - TEM images and processing files for succinic-acid derivative | |-- TEM_D81-16.pptx - assembled TEM figure/slide material -------------------------------------------------------------------- FILENAME STRUCTURE -------------------------------------------------------------------- The raw-data package does not use a standardized repository naming convention. It retains original laboratory, instrument, and figure-preparation filenames. The following interpretation should be used. *Article/sample names: F-PEG-NH2 (1), F-PEG-Gln (2), F-PEG-GA (3), F-PEG-SA (4), F-PEG-FA (5), F-PEG-FITC (6), and F-PEG-Cy7 (7). *Common raw-folder labels: 17F-Peg11_NH2 or 17F-NH2 = amine derivative (1); 17F-Peg11_Gln or 17F-Gln = glutamine derivative (2); 17F-Peg11_GluA or 17F-GluA = glutamic-acid derivative (3); 17F-Peg11_SucA or 17F-SucA = succinic-acid derivative (4); 17F-Peg11_FA or 17FP-FA = folic-acid derivative (5); 17F-Peg11_FITC or 17F-FITC = FITC derivative (6). *NMR folders: AK_21_17F-PEG-GLn odchraneny_Necisteny/43 and /50- siteny II x, AK_22_17F-PEG-Folic acid/44, and AK_23_17F-PEG-FITC/45 follow the Bruker experiment-directory structure. Important files include fid (raw free induction decay), acqu/acqus (acquisition parameters), pulseprogram, audit trails, and pdata/1 (processed spectra). *Confocal files: .oib files are Olympus native image containers; .tif files are exported images. Replicate/field labels such as a, b, c, d and labels such as DAPI, probe, FITC only, or Image0004 describe microscope fields or channels/conditions as recorded by the experimenter. *TEM files: names such as D81-16_033_v1466r_21kxm-1.png or D81-16_024_67kx.tif encode microscope run/image number and magnification; processing/ subfolders contain exported or processed PNG/TIF copies. *Flow-cytometry workbooks: sheet names such as floreadastats_4T1, floreadastats_EL4, floreadastats_LL2, floreadastats_Mcf7, floreadastats_MDA MB231, and floreadastats_Panc02 correspond to cell lines. Rows reference source FCS files by filename, but the native FCS files themselves are not included in the supplied archive. *XTT workbooks: sheet names 4T1, EL4, LL2, Panc02, and Panc02-related worksheets correspond to cell lines and contain absorbance-derived viability summaries and plotting data. *In vivo folder name pankrac_clanek is retained from the original Czech laboratory/author naming; metodika.txt and vysledky.txt mean methods and results, respectively. -------------------------------------------------------------------- FILE TYPES & FORMATS, SW TO OPEN AND DIMENSIONS & UNITS -------------------------------------------------------------------- Bruker NMR directories original format: Bruker NMR experiment folders with fid, acqu/acqus, pulseprogram, audit trails, and pdata/1 processed files SW: Bruker TopSpin 4.0.6 or newer; raw data may also be inspected with compatible NMR software such as MestReNova/Bruker tools horizontal axis: chemical shift, delta (ppm) vertical axis: signal intensity (a.u.) NMR/figure images formats: .png exports of assigned spectra SW: standard image viewer; ImageJ/Fiji; Microsoft PowerPoint/Word as needed units: plotted chemical shift (ppm) and intensity annotations as embedded in the image OriginPro projects formats: .opju files for FTIR, CMC, pKa, DLS/ELS, and biological figures SW: OriginPro. If OriginPro is unavailable, ask the data depositor to export sheets/graphs to CSV/PDF. units: depend on project; usually fluorescence intensity (a.u.) vs concentration (uM), pH vs titrant addition, wavenumber (cm^-1), DLS size (nm), PDI (unitless), zeta-potential (mV), or absorbance/viability (%) Excel workbooks formats: .xlsx and legacy .xls SW: Microsoft Excel, LibreOffice Calc, or compatible spreadsheet software units: CMC in uM; pKa/pH unitless with titrant volume/time as recorded; flow cytometry MFI/median FITC or B525-A in arbitrary fluorescence units and fold over control; XTT absorbance and percent viability; colocalization Pearson/Manders metrics unitless Olympus confocal microscopy files original format: .oib Olympus image binary files SW: Olympus cellSens/Olympus Viewer, Fiji/ImageJ with Bio-Formats, or other Bio-Formats-compatible software auxiliary format: exported .tif images dimensions/units: pixel dimensions and channel metadata stored in .oib; fluorescence intensity in arbitrary units; colocalization metrics reported in Coloc_KeyMetrics_with_FITC.xlsx TEM images and figure files original/auxiliary formats: .tif and .png images, plus TEM_D81-16.pptx assembled figure file SW: ImageJ/Fiji, standard TIFF/PNG viewers, Microsoft PowerPoint/LibreOffice Impress dimensions/units: scale bars and magnification embedded in images; nanoparticle dimensions reported in nm in article/figure data In vivo imaging and graphical files formats: .png and .psd figure/table files, .txt method/result notes SW: standard image viewer, Adobe Photoshop or compatible PSD viewer, Fiji/ImageJ for ROI inspection when image data permit dimensions/units: time after injection (10 min, 1 h, 2 h, 4 h, 24 h, 48 h), fluorescence intensity in arbitrary units/ROI, anatomical labels Li = liver, S = spleen, K = kidney, I = intestine, Ly = lymph node according to tabule_popis.txt -------------------------------------------------------------------- FUNDING -------------------------------------------------------------------- * Ministry of Education, Youth and Sports of the Czech Republic - INTER-EXCELLENCE: Novel Radiosensitizers for FLASH-RT Therapy (LUAUS24203) * Ministry of Education, Youth and Sports of the Czech Republic - INTER-ACTION: Czech National Node to the European Infrastructure for Translational Medicine (LM2023053 EAT- RIS-CZ ERIC) * Ministry of Health of the Czech Republic: Polymer-based supramolecular radiosensitizers for anticancer radiotherapy (NW24-03-00387) * Czech Science Foundation: Macromolecular platform for selective membrane receptor degradation (GA24-10814S) * Ministry of Education, Youth and Sports of the Czech Republic: ″New Technologies for Translational Research in Pharmaceutical Sciences/NETPHARM″ (CZ.02.01.01/00/22_008/0004607), cofunded by the European Union.