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A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI-SNF complex component

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NIAID Data Ecosystem2026-03-12 收录
下载链接:
https://www.omicsdi.org/dataset/pride/PXD022620
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资源简介:
Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-276A) that is commonly used as a probe for NCAPH2, the kleisin subunit of condensin II, in mammalian cells. We find that, in addition to its intended target, this antibody cross-reacts with one or more components of the SWI-SNF family of chromatin remodelling complexes in an NCAPH2-independent manner.
创建时间:
2020-12-14
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