Tumor suppressor REST/NRSF controls genome stability by preventing R-loop formation

Analysis of HeLa cell with overexpression of an EGFP-tagged dominant-negative truncation (73- 563 a.a.) of human REST/NRSF. RNA polymerase II and DNA damage repair factor ?-H2AX binding data provide insight into the molecular bases of how REST/NRSF perturbation impairs genome stability. Overall design: Identification of RNA polymerase II and ?-H2AX binding sites in normal and REST-disrupted HeLa cells. An antibody targeting the YSPTSPS repeats in the C-terminal domain (CTD) of the largest subunit of RNAP II was utilized to immunoprecipitate RNAP II, regardless of its phosphorylation status.