The effect of auxin exposure on the adult Drosophila transcriptome

Auxin is used to induce transgene expression or degrade proteins in Drosophila. Here we exposed three different genotypes to 0 mM or 10 mM auxin and measured changes in gene expression for both males and females. We found that auxin exposure significantly alters the transcriptome of adult Drosophila in all genotypes tested. To test the affects of auxin on the transcriptome, we fed 4-5 day old adult Drosophila to 0 mM or 10 mM auxin for 48 hours. We then processed whole body samples for RNA extraction and sequencing.