Gene expresison of effector and exhausted CD8+ T cells from viremic people living with HIV

The differential expression of metabolic genes between effector and exhausted CD8+ Tcells has been characterized in the people living with HIV (PLWH) using 768 genes from the NanoString Human Metabolic Pathways Panel. Seven T cell subsets were used for the comparison of metabolic landscape. Naïve CD8+ T cells were isolated from healthy controls, while different stages of effector memory, effector, and exhausted CD8+ T cells were purified from viremic PLWH. RNA were harvested from the purified cells, quantified and prepared for the gene expression analysis using Nanostring Human Metabolic Pathways Panel.