Individual actograms of a wild type (A) and Hrh3-/- (B) mice.

Animals were housed for one week under LD12/12, then kept in constant darkness for two weeks and then finally for one week under LD12/12. The shaded area corresponds to a darkness period. Analysis of locomotor activity with χ2-periodogram (D and E). Periodograms are plotted as 95% CI of means of a wild type (D) and knockout (E) mice under LD12/12 (light gray) or constant darkness (dark gray, DD) conditions, horizontal solid line—Qp value at p = 0.01; n = 11 (wild type), 19 (Hrh3-/-). Cosinor analysis of locomotor activity under LD12/12 (D, upper panel) and DD (D, lower panel) regimes: black solid line shows averaged locomotor activity over 48 h, the black dashed line indicates the cosine fit of the averaged activity, light grey area represents the 95% CI of average activity. Analysis of activity fragmentation (E) under LD12/12 (upper panel) and DD (lower panel) regimes of wild type (light gray circles) and Hrh3-/- (black circles) animals.