RNA Expression Profiling Of Normal Mouse Live Across a Reproductive Cycle

Purpose: To perform RNA-Seq expression profiling of the normal rodent liver at nulliparous, lactation, and involution day 6 reproductive stages. Methods: RNA was extracted and isolated from flash frozen murine liver using the Direct-zol RNA MiniPrep kit. An input of 100ng of RNA was used for library preparation. Library construction was performed by Novogene using a NEBNext® Ultra RNA Library Prep Kit for Illumina. Qualified libraries were sequenced on an Illumina Novaseq6000 Platform using a paired-end 150 run. Results: We identified gene pathway differences bile acid production and FGFR4 signaling between different reproductive stages with upregulation of these pathways during lactation. Additionally, individual genes involved with promoting bile acid synthesis were elevated at lactation whereas genes associated with inhibiting bile acid synthesis were reduced during lactation and elevated during involution. Mouse liver derived mRNA from 7 nulliparous mice, 6 lactation stage mice, and 6 involution day 6 mice generated by 150-cycle paired-end sequencing using the Illumina Novaseq6000 Platform. Fastq files were aligned to mm10 mouse reference genome (GRCm38.89) and per-gene counts quantified by RSEM (version 1.3.1) based on the gene annotation Mus_musculus.GRCm38.89.chr.gtf. Differential gene expression analysis was performed using DESeq2 (version 1.22.2).