Multi temperal single-cell profiling decodes cross-talk of ?d17 T cells and neutrophils in radiation pneumonitis

Radiation pneumonitis (RP) is a common and fatal complication in thoracic radiation therapy. The immune microenvironment plays an important role in the RP development. However, the full scope of radiation immune changes over time remains unclear. In this study, a murine model of RP was established, and distinct early (10 days) and late phases (100 days) were defined. We conducted single-cell RNA sequencing and related RNA-seq and proteomics to explore the underlying mechanisms of persistent inflammation during RP. By examining the immunological effects of RP over time at the single-cell level, we shed light on its dynamic immunological effects of RP over time at single-cell resolution and provided potential therapeutic targets for clinical interventions. Overall design: C57BL/6N female mices were analysed after being treated either with a single dose of 20 Gy of 9 MeV electron-ray or with no treatment from 10 days or 100 days post-radiation.