Transcriptomic analysis to identify genes associated with hypothalamus vulnerability in aging mice with cognitive decline

Normal aging process is accompanied by cognitive decline. Studies have shown that the hypothalamus plays an important role in regulating aging and cognition. However, the exact molecular mechanism remains unclear. Therefore, the present study aimed to identify predictors of aging cognitive decline in the hypothalamus. The behavioral experiment was used to identify learning and memory differences between young and old mice. Transcriptome sequencing was performed on the hypothalamus of young and old mice to identify potential genes.Our data provide the related genes ,which may be potential predictors of cognitive function in the elderly population, which will establish an important foundation for us to further explore the molecular mechanism. To identify predictors of aging cognitive decline in the hypothalamus, five young mice (12 weeks old) and five aging mice (22 months old) were used to establish animal models.After the behavioral experiment, three of each group were randomly selected for transcriptome sequencing.正常衰老过程伴随认知功能减退。研究表明，下丘脑（hypothalamus）在调控衰老与认知进程中发挥关键作用。然而其确切的分子机制仍未阐明。为此，本研究旨在筛选下丘脑中与衰老相关认知减退的预测因子。本研究通过行为实验甄别年轻与老年小鼠的学习记忆差异，并对两组小鼠的下丘脑组织开展转录组测序（transcriptome sequencing）以筛选潜在差异基因。本研究所得数据涵盖相关差异基因，这些基因或可作为老年人群认知功能的潜在预测因子，为后续深入探究其分子机制奠定重要基础。为筛选下丘脑中衰老相关认知减退的预测因子，本研究构建了5只12周龄年轻小鼠与5只22月龄老年小鼠的动物模型。行为实验结束后，每组随机选取3只小鼠，对其下丘脑组织进行转录组测序。