Spatial transcriptomics of fixed tissue micro-regions using Pick-Seq [tonsil]
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https://www.ncbi.nlm.nih.gov/sra/SRP285373
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资源简介：
Spatial transcriptomics and multiplexed imaging are complementary methods for studying tissue biology. Here we describe a simple method for transcriptional profiling of formalin fixed histology specimens based on mechanical isolation of tissue micro-regions containing 5-20 cells. Sequencing micro-regions from an archival melanoma specimen having multiple distinct histologies reveals significant differences in transcriptional programs associated with tumor invasion, proliferation, and immunoediting and parallel imaging confirms changes in immuno-phenotypes and cancer cell states. Overall design: mRNA from 40µm regions of interest were collected from two histological sites in human FFPE tonsil sections, sequenced and gene expression profiles were analyzed.
创建时间：
2021-09-03
