Biofilm Data

Biofilm Data.csv, Data description: Data used to create Figure 3: Biofilm production of evolved strains. Results show the average biofilm production of 6 isolates evolved in each experimental environment, relative to the ancestral strain from which all evolved strains were derived. Data was collected in the laboratory using a crystal violet staining method. Columns correspond to: Strain Replicate: the name allocated to each of the replicate lines, e.g. H1 corresponds to replicate line 1 evolved on hard agar. Bioflim Replicate: The biofilm production was measured independently eight times in order to provide replicates. This value was averaged in statistical analysis. Agar Type on which Strain was Evolved: Strains were either evolved in hard or liquid agar. Biofilm Relative to Ancestor: Vmax of evolved strain under each treatment group was divided by Vmax of ancestral strain under the same treatment to give a relative measurement of area dispersed. Averaged Biofilm Replicates: Replicate relative biofilm measurements were averaged. Selection Type: Strains were either under positive or neutral artificial selection for dispersal.

Biofilm Data.csv，数据说明：本数据集用于构建图3：进化菌株的生物膜生成能力。实验结果展示了各实验环境下6株进化分离菌株的平均生物膜生成量，以所有进化菌株的起源祖先菌株作为参照基准。实验数据通过实验室结晶紫染色法采集获得。各列对应信息如下：
1. 菌株重复株（Strain Replicate）：为各重复菌株系分配的编号，例如H1代表在硬琼脂上进化得到的第1号菌株系。
2. 生物膜重复测量（Biofilm Replicate）：为设置重复组以保证实验可靠性，生物膜生成量被独立测定8次，统计分析时对该组测量值取平均。
3. 菌株进化所用琼脂类型（Agar Type on which Strain was Evolved）：菌株分别在硬琼脂或液体琼脂中完成进化培养。
4. 相对于祖先菌株的生物膜生成量（Biofilm Relative to Ancestor）：将各处理组下进化菌株的Vmax值，除以同一处理组下祖先菌株的Vmax值，得到相对的生物膜扩散面积测量值。
5. 生物膜重复测量平均值（Averaged Biofilm Replicates）：对多次重复的相对生物膜测量值取平均所得结果。
6. 选择类型（Selection Type）：菌株的扩散性状分别接受正向人工选择或中性人工选择。