Single cell RNA sequencing on PyMT mammary gland primary tumor and lung metastases

PyMT-GPx2 KD tumor cells were GFP-labeled in vitro and then injected into mammary fat pad of mice for incubating 45 days. We then harvested GPx2 KD primary tumor and lungs and generated single cell suspension by FACS sorting from GPx2 KD, and paired lung metastases. 10X Genomics was used to make cDNA library. We then sequenced the samples with Illumina high throughput sequencing.