Small RNA profiling of differentiated and de-differentiated vascular smooth muscle cell. Small RNA profiling of differentiated and de-differentiated vascular smooth muscle cell

We report the application of small RNA sequencing for high-throughput profiling of small RNA under 75 bp in vascular smooth muscle cell. By a reading depth of 30M and single stranded sequencing, we generated the small RNA signature on differentiated and de-differentiated vascular smooth muscle cell induced by PDGF-BB and H3K4me2 editing. We found that PDGF-BB and H3K4me2 editing induced de-differentiation modulated miRNA profile significantly, which was demonstrated at least in part responsible for modulated vascular smooth muscle cell phenotype. Overall design: Profiling of differentiated and PDGF-BB and H3K4me2 edited de-differentiated vascular smooth muscle cell