Next Generation Sequencing Facilitates Quantitative Analysis of Drosophila larval Wild Type and TFAM RNAi (r4-gal4>TFAM RNAi) Fat body Transcriptomes

Purpose: The goal of this RNA-Seq is to analyze the transcriptomes from wild type and TFAM RNAi fat body samples at 96 hr AEL (after egg laying) and to identify the differentially expressed genes. Methods: Drosophila larval fat bodies were isolated from wild type and TFAM RNAi (r4-gal4) animals at 96 hrs AEL (after egg laying) of development. mRAN profiles were generated by Next Generation Sequencing Facilities. Results: RNA Seq differential expression analysis identified a significant change in 1758 transcripts, with 1213 transcripts showing reduced expression levels and 545 transcripts with elevated expression levels. Drosophila larval fatbody mRNA profiles of wild type and TFAM RNAi