Effects of peptides on vertebrate tissues

aIn convention with IUPAC-IUB, Biochemistry 1967, 6(1), 362-368.
bPercent purity determined by RP-HPLC using buffer systems: A = 0.1% TFA/H2O and B = 60% CH3CN/0.1% TFA in H2O with a gradient slope of 3% B/min, at flow rate of 1,5 ml/min on a Beckman C18 column (4,6×150 mm, 5 μm particle size, 300 Å pore). Determination at λ = 214 nm.
cCZE was done using a Waters System, model CIA (Capillary Ion Analyzer) by hydrostatic injection in 25 seconds, utilizing Phosphate buffer pH 2,50, silica capillary (75 μm ID×60 cm length), field strength of 20 kV at temperature 30°C. Determination at λ = 214 nm.
dMolecular weights were determined by Maldi-TOF using a α-ciano-4-hydroxicinamic acid matrix.
eBiological activities of VC1-6 were compared to those of angiotensin II on the guinea pig ileum, rat uterus, rat blood pressure and chicken ileum. The hemolytic activity of angiotensin II and its analogues was measured at a peptide concentration ranging from 0.19 to 100 μM.