Supplement 7 ASR

We test the repressive functions of ancestrally reconstructed LisH-H1 sequences. We also test the functions of full length LisH sequences (unpublished).This allows us to further test evolutionary evidence of a conserved repressive LisH-H1 function.
Conclusion:
LisH-H1 repressive function is conserved and ancestral. This data also further provides evidence of amino acids that give lisH H1 its identity. We also see that full length LisHs are fully repressive in this circuit. We have a negative control (no repression alpha helix control, and no repression noIAA3 and no H1 control) and a positive control (strong repressor TPL H1). We gate for live haploid yeast cells. We dilute in the morning and wait to log growth to make fluorescence measurements.