Transcriptomic analysis in hippocampus of C57BL/6 and DBA/2 mice

Background: Previous studies have shown that significant difference in learning and memory ability existed between C57BL/6 and DBA/2 mouse. Identification of behavioral and genetic differences between these two strains is typically important for neuroscience research, indicating that they can be used to identify candidate genes for learning and memory. Objective: This study was conducted to identify candidate genes for learning and memory using C57BL/6 and DBA/2 mouse. Methods: We conducted a transcriptomic analysis in hippocampus of C57BL/6 and DBA/2 mouse using high throughput RNA deep sequencing (RNA-seq) technology to detect differentially expressed genes (DEGs). Differentially expressed genes were then functionally annotated. Results: We found a total of 31,714 genes from hippocampi of C57BL/6 and DBA/2 mice, which comprised 4,241 to 4,599 known genes with new isoforms and, 4,023 to 4,451 structurally optimized known genes. Among these genes, a total of 458 genes showed different expression in the two strains of mouse. Eight genes were randomly selected to be validated by qRT-PCR, and the expression levels of all genes were consistent with the RNA sequencing. Conclusion: Our data indicated that there were different gene expression patterns between hippocampi of C57BL/6 and DBA/2 mice. The genes associated with learning and memory discovered in the study may enhance the explanation of the behavioral differences between the two mice. We conducted a transcriptomic analysis in hippocampus of C57BL/6 and DBA/2 mouse using high throughput RNA deep sequencing (RNA-seq) technology to detect differentially expressed genes (DEGs).背景：既往研究表明，C57BL/6与DBA/2两种小鼠在学习与记忆能力上存在显著差异。对这两种品系小鼠的行为与遗传差异进行鉴定，是神经科学研究的重要方向，这也提示二者可用于筛选学习记忆相关的候选基因。 目的：本研究旨在利用C57BL/6与DBA/2小鼠，筛选与学习记忆相关的候选基因。 方法：我们采用高通量RNA深度测序（RNA-seq）技术，对C57BL/6与DBA/2小鼠的海马组织进行转录组分析，以筛选差异表达基因（DEGs），随后对差异表达基因进行功能注释。 结果：本研究从C57BL/6与DBA/2小鼠的海马组织中共鉴定出31714个基因，其中包含4241至4599个带有新转录本的已知基因，以及4023至4451个经过结构优化的已知基因。在这些基因中，共有458个基因在两种品系小鼠中呈现表达差异。我们随机选取8个基因通过实时定量聚合酶链反应（qRT-PCR）进行验证，所有基因的表达水平均与RNA测序结果一致。 结论：本研究数据表明，C57BL/6与DBA/2小鼠的海马组织存在不同的基因表达模式。本研究中发现的与学习记忆相关的基因，有助于进一步阐释两种小鼠的行为差异机制。我们采用高通量RNA深度测序（RNA-seq）技术，对C57BL/6与DBA/2小鼠的海马组织进行转录组分析，以筛选差异表达基因（DEGs）。