Genome-wide identification of 5hmCpGs in multiple myeloma

We report the genomic localization of 5-hydroxymethylcytosines in multiple myeloma patients through the use of selective chemical labeling (SCL) and exonuclease digestion. Data highlighted super-enhancers and enhancers specifically undergoing active DNA demethylation in multiple myeloma cells. Genomic DNA from multiple myeloma (MM) cells was extracted and submitted to the SCL-exo protocol. After sequencing with a HiSeq 1500 (Illumina), reads were mapped to hg19 and processed to generate a single-CpG signal file (wig file) indicating the relative levels of hydroxymethylation.