Transcriptomic cell types of ventromedial hypothalamus in female mouse: 10x cells

The ventrolateral subdivision of the ventromedial hypothalamus (VMHvl) is essential for innate social behaviors including aggression and mating in female mice at different reproductive states. However, the female VMHvl transcriptomes in different reproducive states and their relationship behavioral functions are unknown. We performed single-cell RNA 10x sequencing and investigated correspondence between transcriptomic identity in different reproductive states and behavioral activation. Immediate early gene analysis from our Act-seq dataset identified that distinct T-types function in female aggression and mating behaviors. Single cell characterization of VMHvl in virgin, lactating and post-lactation female mice腹内侧下丘脑腹外侧亚区（ventrolateral subdivision of the ventromedial hypothalamus, VMHvl）对不同生殖状态雌性小鼠的先天社交行为（包括攻击与交配）至关重要。然而，目前不同生殖状态下雌性VMHvl的转录组特征及其与行为功能的关联仍不明确。本研究开展了10x单细胞RNA测序，探究了不同生殖状态下转录组特征与行为激活之间的对应关系。通过对本研究的Act-seq数据集进行即刻早期基因分析，我们发现不同的T细胞亚型在雌性小鼠的攻击与交配行为中发挥独特功能。本研究对未交配、泌乳期及泌乳后期雌性小鼠的VMHvl开展了单细胞表征。