Genomic landscape of the developing kidney upon conditional and time-dependent knockout of Wt1

We are characterizing global expression in the whole kidney at developmental stage E18.5, where we have used three Cre strains that result in knock out Wt1 in different cell types during renal development. Here the purpose is to identify the developmental stage at which the loss of Wt1 impacts kidney development, specifically in the context of a degree of disruption capable of ending in pediatric kidney cancers. Total RNA was isolated from whole kidneys of E18.5 mouse embryos following a variety of conditional knockouts of Wt1, plus appropriate control animals.本研究针对小鼠胚胎发育阶段E18.5的完整肾脏开展全局基因表达谱特征分析，实验中采用了三种Cre重组酶品系，可在肾脏发育过程中的不同细胞类型内条件性敲除Wt1基因。本研究的核心目标是明确Wt1缺失影响肾脏发育的具体发育阶段，重点聚焦于可引发儿童肾癌的肾脏发育异常程度。我们从经多种条件性Wt1敲除处理的E18.5小鼠胚胎完整肾脏以及对应的对照动物肾脏中分离得到总RNA。