Draft genome assembly of a Japanese Oikopleura dioica male individual (O3), using Nanopore long reads.

This draft assembly was used to validate the chrY scaffolds of the OSKA2016 reference genome in the publication  “A genome database for a Japanese population of the larvacean Oikopleura dioica”, Development Growth and Differentiation, Wang and coll., 2020 (in press). It is provided as supplemental data for the reproducibility of this work; please note that no further polishing has been done to correct sequencing errors.

Genome sequence reads were produced on a MinION sequencer (Oxford Nanopore Technologies) using high-molecular weight DNA from a male individual of the Oikopleura dioica species of zooplankton. The individual was related to the laboratory strain established from a western Japanese population that was used to produce the OSKA2016 reference genome. The raw reads were basecalled with the Guppy software version 3.3.0 using its dna_r9.4.1_450bps algorithm, and deposited in the European Nucleotide Archive (Study ID: PRJEB38559). The draft assembly was made with the Flye software version 2.7 with the options --genome-size 65m and --min-overlap 3000.