Acidosis activates the Nrf2-pathway in renal proximal tubule-derived cells through a crosstalk with renal fibroblasts

Crosstalk of renal epithelial cells with interstitial fibroblasts plays an important role in kidney pathophysiology. A former study showed that crosstalk between renal epithelial cells and renal fibroblasts protects against acidosis-induced damage. In order to gain further mechanistic insight into this crosstalk we investigated the effect of acidosis on the transcriptome of renal epithelial cells (NRK-52E) and renal fibroblasts (NRK-49F) in co-culture by RNASeq, bioinformatics analysis and experimental validation.肾上皮细胞与间质成纤维细胞的串扰在肾脏病理生理过程中发挥关键作用。既往研究显示，肾上皮细胞与肾成纤维细胞之间的串扰可抵御酸中毒诱导的细胞损伤。为深入解析该串扰的分子机制，本研究通过RNA测序（RNASeq）、生物信息学分析及实验验证，探究了酸中毒对共培养体系中肾上皮细胞（NRK-52E）与肾成纤维细胞（NRK-49F）转录组的影响。