Primers and amplification program used in the study.
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The amplification program was as follows: 95°C/15min, followed by 47 cycles of denaturation at 94°C/15sec, annealing Ta* at 25sec or 30sec, extension at 72°C/15sec. Melting curves for each specific primer were monitored between 60°C–90°C, at the end of amplification, before a further extension at 75°C/5min. In bold, referring gene details;*Ta, annealing temperature; bp, base-pairs of amplicons.Primers and amplification program used in the study.
创建时间：
2015-12-03
