Sixteen nicotiana species Raw sequence reads. Nicotiana

we performed the RNA-seq and de novo assembly of leaf transcriptome of 16 Nicotiana species. We identified the phylogenetic relationship of these Nicotiana species on the transcriptome levelusing the Maximum Likelihood method.Furthermore, We revealed the expression patterns and correlations of genes in leaves related to the biosynthesis and degradation of nicotine, respectively.This is the first report on the leaf transcriptomes of sixteen Nicotiana species by RNA-seq and de novo assembly. Our results not only validate and improve the phylogenetic relationships of Nicotiana species, but also lay a foundation for analyzing key genes in leaves involved in the pathway of nicotine.