The S. cerevisiae m6A reader Pho92 promotes timely meiotic recombination by controlling key methylated transcripts [RNA-seq]

We performed high-throughput mRNA sequencing during a meiotic time course (0, 2, 3, 5, 6, 7 and 9 hr) in WT, pho92 mutant and ime4 mutant cells. Meiosis was induced by resuspension of cells in SPO medium and high synchronicity was achieved thanks to the SK1 pCUP-IME1 background alowing inducible activation of IME1 after 2 hr in SPO by addition of 50 µM of copper (II) sulfate. PolyA RNAs were extracted from cells collected at 7 time points from 3 different strains (WT, pho92 mutant, ime4 mutant) and in 3 biological replicates, giving a total of 63 yeast mRNA-seq samples.