five

E. coli Affinity purification MS/MS

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NIAID Data Ecosystem2026-03-12 收录
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https://www.omicsdi.org/dataset/pride/PXD013749
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The tRNA linked repeat sequence rrB was tagged in either 5'- or 3'- end with the MS2 aptamer and expressed from a plasmid in E. coli. Using the MS2 aptamer, the RNAs were affinity purified after cell lysis. The co-purifying proteins were identified by NanoLC-MS/MS and compared to the proteins co-purifying with the MS2 sequence alone.
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2021-04-26
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