LC-MS/MS for identifying the ISGylated sites of DPP3
收藏国家人口健康科学数据中心2026-06-01 收录
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https://www.ncmi.cn/phda/dataDetails.do?id=CSTR:17970.11.A001G.202512.45.V1.0
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资源简介：
To explore the ISGylated sites of DPP3. DPP3-Flag and the ISGylation system containing UBA7, UBE2L6, and ISG15 were co-transfected in HEK293T cells. Cells were harvested after 48 hours and lysed, then co-immunoprecipitation with anti-Flag antibody magnetic beads. The sample was verified by western blot. Then the sample was electrophoresed in polyacrylamide gel and LC-MS/MS was performed using gel containing Co-IP sample to identify the ISGylation sites of DPP3.
提供机构：
北京蛋白质组研究中心
创建时间：
2024-11-17
