Gene expression differences between activated B cells expressing GL7 and not in Peyer´s patches. Gene expression differences between activated B cells expressing GL7 and not in Peyer´s patches

We report two populations of activated germinal centre B cells in the Peyer´s patches that are distinguished by their expression of GL7 or not. Using an antigen-specific system, we sort GL7+ and GL7- activated cells and performe RNASeq. We also included GL7 expressing B cells activated against the gut microbiota, and resting B cells from Peyer´s patches and spleen. Overall design: GFP expressing NP-specific B cells from spleen were transfered into recipient wild type C57Bl/6 mice and the mice were orally immunized with NP conjugated to cholera toxin (NP-CT). Antigen-specific cells were isolated based on GFP and GL7 expression and compared to endogenous GL7+ and GL7- cells.